The Entomological Society of Iran Journal of Insect Biodiversity and Systematics 2423-8112 12 3 2026 09 01 Insight into the molecular diversity of Perreyia flavipes, a comparison across its geographic distribution limits in South America 567 575 28697 10.48311/jibs.12.03.567 EN Matías Feijoo Departamento de sistemas agrarios y paisajes culturales (DSAyPC), Centro universitario de la Región Este (CURE), Universidad de la República (UdelaR). Ruta 8, km 282, Treinta y Tres, Uruguay. 0009-0004-8319-8194 Jorge Escalona Departamento de sistemas agrarios y paisajes culturales (DSAyPC), Centro universitario de la Región Este (CURE), Universidad de la República (UdelaR). Ruta 8, km 282, Treinta y Tres, Uruguay. 0009-0008-6214-6343 Ana Cecilia Corro Centro universitario de la región noreste sede Cerro Largo (CUCEL), Universidad de la República (UdelaR). Luis Alberto de Herrera 639, Melo, Uruguay 0000-0003-0967-993X Mauro Pereira Soares Laboratório Regional de Diagnóstico, Faculdade de Veterinária, Universidade Federal de Pelotas (UFPEL). Pelotas, Rio Grande do Sul, Brazil 0000-0001-7427-140X Fernando Dutra División de Laboratorios Veterinarios "Miguel C Rubino", Laboratorio Regional Este (DILAVE), Ministerio de Ganadería, Agricultura y Pesca (MGAP). Avelino Miranda 2045, Treinta y Tres, Uruguay 0000-0003-4886-9429 Journal Article 2025 11 25 Perreyia flavipes Konow, 1899 is one of the fairly known species of the Pergidae family. The larval stage of the species presents a high incidence of intoxication outbreaks in South America with an important economic impact on cattle, sheep, and pig production. Examined specimens were collected from the southern and northern extremes of the species distribution. Southern-limit specimens from Uruguay and Brazil were used to amplify and sequence COI, 16S, and 28S genes, and the same gene sequences were obtained from the Genbank database for the northern limit in Colombia, together with three other species of the Pergidae group. High similarity between Brazilian and Uruguayan samples was found. Colombian haplotypes were highly differentiated from those of the Uruguay-Brazil group. Paired differences between Perreyia species were similar to those found between southern and northern comparisons of P. flavipes, suggesting the need to deeply understand and describe the incipient differentiation of the species across its wide geographic distribution. https://jibs.modares.ac.ir/article_28697_46a62c34c7b8b0c0d02f0833df49ec20.pdf