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Aims: Adolescence is one the most precarious periods of life, concerning the drug abuse. The social cost of the drug abuse and injury among adolescents is extraordinary and requires intervention. The theory of planned behavior (TPB) is perhaps the most influential theory for prediction of social and health behaviors including drug abuse. This study aimed at designing and implementing a curriculum based on the TPB for preventing adolescents from drug abuse. Methods and Materials: This quasi-experimental study was conducted in Hamadan, west Iran. We recruited 140 male high school students from randomly selected schools: they were divided into experimental group, n = 70 and control group, n = 70. The experimental group received 20 hours educational program based on the TPB. The control group receives no intervention. Findings: Compared to the control group, experimental group have significantly elevated post test scores for attitude (19.07 vs. 15.28, p < .001), subjective norm (18.08 vs. 16.45, p < .001), perceived behavioral control (51.67 vs. 54.82, p < .001); and their intention to use drug significantly decreased(p = 0.082) Conclusions: The TPB-based educational program may be effective in prevention of substance abuse among adolescents.

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Aim: Tobacco use is one of the major causes of death worldwide and the most preventable risk factor of the coronary heart disease. The purpose of this study was to determine attitudes, risk perception and perceived vulnerability toward to water pipe (WP) smoking among male students in Zarandieh city of Iran. Methods: This cross-sectional descriptive study was carried out on 400 male adolescents in 2011-2012. A multiple-stage sampling protocol was used. The participants completed a self-report questionnaire about demographics, knowledge, attitude and beliefs about WP smoking and their tobacco use background. Data were analyzed by SPSS16. Findings: The prevalence of WP smoking was 72 (18%). Also 50% of participants believed water pipe smoking is less harmful compared to cigarettes. The mean score of knowledge, attitude, and risk perception for non smokers was higher from smokers (knowledge 4.9±2.1, attitude 19.5±4.3 and risk perception 18.6±3.3 versus 3.4±1.5, 15.6±5.5 and 15.4±4.3 respectively), whereas smokers reported a fairly high level of perceived stress and depression (perceived vulnerability). Conclusion: Noticing the side effects of tobacco use and the high prevalence of incorrect perceived WP smoking beliefs among students, provision of educational programs for the correction of the beliefs of students regarding WP smoking is recommended.

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Aim: Benefits of Skin-to-Skin Contact (SSC) between mother and her newborn, specifically when it is conducted immediately after birth, have been recognized for more than 40 years. To determine why SSC is not conducted, the present study considers the opinions of the labor working midwifes about the reinforcing factors in SSC immediately after birth in Tehran's hospitals in 2012-2013. Methods: In this descriptive study, we have employed the reinforcing factors of the phase 3 of PRECEDE-PROCEED model. The samples consisted of 292 midwifes who were responsible for delivery or for newborns immediately after birth in 18 hospitals of Tehran. The sampling was firstly performed using stratified and then simple random manner. They were classified into educational, social security, and private hospitals. Data collection instrument was a self-developed questionnaire consisting of demographic characteristics, social support and midwifes' motivation to conduct the reinforcing factors in the SCC at birth. It was derived from a qualitative study. Internal consistency of the questionnaire was assessed using Cronbach's alpha coefficient. Data analysis was conducted through applying the SPSS version 18. Findings: The results show that 93.8% of midwifes had a good idea about social support of the midwifes with Cronbach's alpha 0.744 and 96.6% of them believed in the Midwifes ' motivation in skin contact effects with Cronbach's 0.773. Conclusions: The midwives believed in the key role of reinforcing factors such as social support and their own motivation in successful and standard SSC. Therefore, further studies are suggested on exploring the opinions of pediatric, obstetrics and anesthesia specialists, midwives, mothers and their husbands concerning the SSC.

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Aim: Promotion of oral health in schools is one of the recommended polices of WHO. The aim of this study was to determine the role of Oral Hygiene Beliefs according to the Health Belief Model in regular brushing behavior, among the 9-10 years old female students. Methods: This cross sectional study was conducted on the female students of Rasht-Iran in 2012. Applying the statistical estimation, the sample population was determined 265 who were selected from 22 schools using systematic sampling method. Nearly 12 students were selected from each school using simple randomized method. Data collection instrument was a questionnaire and the results were analyzed using descriptive methods, and analytical methods including Chi-square, Mann-Whitney U test and Logistic regression. Finding: Among the samples, 17.4% brushed at least twice a day. The logistic regression analysis indicated that perceived severity (OR = 0.48, 95% CI = 0.27- 0.86, P = 0.014), perceived barriers (OR = 1.97, 95% CI = 1.29- 3.02, P = 0.002) and mothers' educational level (OR = 4.78, 95% CI = 1.24- 18.46, P = 0.023) were the significant predicting factors for tooth brushing twice a day or more. There are significant statistical correlations among the perceived severity of loosing good eating and good speaking, and perceived barriers of tiredness, laziness and impatience with desirable behavior. Overall 66.5% agreed with one of the two physical barriers: painful tooth brushing and gums bleeding. Conclusion: Promoting educational level and educational programs for Iranian women are recommended. Planning useful educational interventions for the students of concrete operational stage is required attention to the application of Piaget’s Theory of cognitive development. Educational interventions should focus on items of beliefs named above. At least 70 percent of Iranian people need to learn the correct ways of brushing teeth.

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Aim: Using short text messages (SMS) is the easiest and cheapest method of health education programs in order to increase women’s’ knowledge and early detection of diseases. This study was conducted in 2013 in order to compare the educational effect of breast self examination (BSE) by two different methods of SMS and lecturing in female students who work and study in non- medical fields. Method: In a quasi-experimental study, 108 twenty- two to thirty year old female students of non- medical fields were chosen by random cluster sampling and then divided into 2 groups. One group was educated by lecturing and the other by SMS. Data collection tool was a questionnaire including demographic, knowledge, attitude and performance sections (KAP) and a check list for direct observation. Collected data were analyzed by SPSS 20 software, using parametric statistical tests (paired T-test) with the significant level of p<0.05. Findings: After education in both groups, a significant difference was observed in the average scores of KAP (p= 0.001). Educating by both lecturing and SMS promoted the level of KAP about BSE (p=0.001). KAP score of doing BS in text message group was higher than the lecture group (p=0.001). SMS was chosen as the best way of BSE education. Conclusion: Compared KAP scores about the quality of doing BSE in the SMS group were higher than the lecture group; therefore, it is suggested that this educating method can be used in the health education programs especially in controlling breast cancer and maintaining the level of health among women in the society.

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Aim: Investigating factors affecting the adoption of preventive behaviors in women with osteoporosis is necessary. This study aims at determining the preventive treatment of osteoporosis in women in Fasa using the Health Belief Model (HBM) during 2014. Method: In this cross-sectional study, 401 women (aged 30-50 years) covered by the health centers in Fasa city were randomly chosen. Construct of HBM and the function of feeding and walking were determined to prevent osteoporosis in women. Findings: The mean age of women was 40/9±6/2 years. The variables of perceived susceptibility (p=0.007), motivation (p=0.009) for walking behavior and variables of perceived sensitivity (p=0.020) for feeding behavior were predicted. Conclusions: This study confirmed the power of HBM in predicting feeding and walking behavior for prevention of osteoporosis. Hence, this model can be used as a framework for designing and implementing educational interventions for prevention of osteoporosis in women.

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The current research is based on the theory of Lucien Goldman The creator of the work of art is the representative of a particular class whose collective thinking turned into a productive work by his author, the comparative-analytical study of the social sources of the poems of Donqle and Hosseini as intellectuals of the popular class. Prodotti explains the presence of collective thought in their mental network by examining the ideals of these two poets. The achievement of this study suggests that both poets have a duty to distinguish and to expand the collective thinking of ideals that are summed up in the land, justice and culture. They succeeded in fulfilling this mission against the society and the people's classes and their lost ideals, and obstacles to achieving these aspirations, which were often the result of malicious foreign policy and, in some cases, the mismanagement of internal leaders. , Identify.

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Background: The importance of this research was to determine the prevalence of efflux pump genes among Acinetobacter baumannii isolates from hospitalized patients in Imam Reza hospital in Tabriz, Iran.
Materials and Methods: This descriptive study was conducted in the Imam Reza hospital, Tabriz, IR Iran during June 2013 to March 2014. Twenty-six strains were isolated from female patients (42.6%) and thirty-five from male patients (57.4%). Clinical specimens were cultured for isolation of the microbial agents of A. baumannii. The isolated bacteria were identified using biochemical tests. Disk diffusion susceptibility test was used to determine the antimicrobial susceptibility, and E-test methods were also used. The prevalence of efflux pump genes was detected by PCR and sequencing methods.
Results: The resistance of A. baumannii isolates against tested antibiotics was analyzed as follows: 51 (84%) to trimethoprim-sulfamethoxazole, 59 (98%) to ceftazidime, 60 (99%) to ciprofloxacin, 29 (48%) to amikacin, 46 (77%) to gentamicin, 30 (50%) to tobramicin, , 60 (99%) to imipenem,, 60 (99%) to meropenem,, 60 (99%) to ceftriaxon,, 60 (99%) to cefepime,, 60 (99%) to ofloxacin, 6 (11%) to colistin. By using E-test, 45 (73.3%) to imipenem, 57 (93.3%) to ciprofloxacin, 23 (38%) to amikacin were also analyzed. The prevalence of adeA, adeB, adeC, and abeMgenes was 54 (88.5%), 61 (100%), 57 (93.9%), and 60 (98.3%), respectively.
Conclusion: The result of this study showed high incidence of AdeABC efflux pump in MDR A. baumannii isolates and the growing number of nosocomial infections associated with XDR A. baumannii complex, leading to difficulties in antibiotic therapy.

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Willow leaf beetle, Plagiodera versicolora is an important pest of willow trees that feeds on the leaves both as larvae and as adults. -Amylases (EC 3.2.1.1) are the major insect digestive enzymes that catalyze the endohydrolysis of long -1,4-glucan chains such as starch and glycogen. In the current study, -amylase activity was studied in the midgut of larvae and adults of P. versicolora. Amylase activity in the midgut of larvae and adults was 0.6807 and 0.1162 µmol/min/mg protein, respectively. The optimal pH for -amylase activity of larvae and adults was 4 and 8, respectively and the optimal temperature for both was 35 °C. The enzyme activity of larvae was inhibited by the addition of Na²⁺, K⁺ and Zn²⁺. K⁺ (at 5 mmol) had the most positive effect on α-amylase activity in adults. EGTA had significant influence on decreasing the enzyme activity in larvae. EDTA had the most effect on increasing the activity of the enzyme in adults. Plant amylase inhibitors play important role against insect pests. Therefore, the characterization of digestive enzymes and the examination of inhibitors on the enzyme activity could be useful in tackling insect pests.    

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Aim: Despite the evidence suggesting the positive impact of the immediate mother-newborn Skin-to-Skin Contact (SCC), it has not yet been adopted to use for healthy newborn in Iran. No study has explained the reasons. This study aimed to survey midwives' opinions about the predisposing factors in SCC at birth in Tehran hospitals in 2014.
Method: The samples, in this cross sectional descriptive study, were 292 midwives who worked in labour wards, and a multi- stage cluster sampling was applied. First we applied stratified and simple random approaches. Then they were classified into educational, social security, and private groups. Data collection instrument was a self- developed questionnaire consisting of 9 demographic characteristics and 38 items concerning the predisposing factors in SCC. We applied face/content validity and item impact method for the instrument's validity. For assessing the internal consistency of the instrument, Cronbach's alpha coefficient was used. All statistical analyses were performed using the SPSS-18.
Findings: The findings showed that 95.5% of the midwives possessed good knowledge of SSC, 93.2% had positive attitudes, 96.6% believed in SSC effects, and 94.9% had a good self-efficacy perception. The Cronbach‟s alpha coefficient of the 38-item instrument showed excellent internal consistency (α=0.88), and it was valid and reliable to measure predisposing factors in SSC.
Conclusion: The knowledge and attitudes of conducting SSC at birth were evaluated at “good” and “positive”, respectively. Therefore, we suggest further analytic studies to determine how these factors could affect on midwife behaviour.

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Teaching every language has got its own special hindrances and features. Undoubtedly, teaching Arabic Language in Iranian schools is not excluded from this fact. Some of the hindrances of learning Arabic language are refered to the content order of the school books. Especially teaching Arabic high language in grade one seems to be a difficult job for some reasons such as the students' enterance to a new stage. If the different components of the books' content get realized and catagorized, the understanding and explication would be easier. Thus analizing the content of Arabic high school book in grade one can be an important step towards identification of its weaknesses. This article aims to evaluate the Arabaic high school book in grade one on the basis of content analysis patterns and arrangement criteria relying on Content Analysis Approach, which is a resaerch method for objective, quantitative and organized discription of superficial contents in communications. The research was descriptive-analytic. The results showed that considering These patterns and criteria, this school book has been highly successful in accurately performing the principles of edunation planning and content arrangement.  

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Instruments and Methods: This cross sectional descriptive-analytical study was conducted on 225 women in reproductive age (15-49) with breast cancer and having a mastectomy referred to Sina Hospital-breast surgery clinic, Breast Research Center, and Mahdieh Hospital from 2014 to 2015. A simple random sampling method as well as 4 questionnaires including the Health Literacy for the Iranian Adults (HELIA), Standard questionnaire of the European Organization for Research and Treatment of Cancer (EORTC version 3.0), special edition of Breast Cancer (QLQ-BR 23), and a Special questionnaire for body images was used. The data were analyzed by SPSS 21 software, using Spearman's correlation coefficient and one way ANOVA test.

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 Discussion and research in different rhetorical and semantic aspects of the Quran, and especially the syntactic and rhetorical aspects, have astonished the Quranic researchers of every age. Among these rhetorical issues is the discussion of the Tanwin and its hidden intentions and purposes. This unique phenomenon of the Arabic language, which is widely used in the Holy Quran, has other secondary meanings that are sporadically mentioned in the syntactic books, in addition to its original meaning (indicating indefinite noun). By carefully reflecting on the Quranic verses, it appears that the Tanwin has other implications such as, amongst other things, magnification, reduction, proliferation, diversification, humiliation, generalization. A field survey of the Farsi translations of the Quran shows that most translators have not paid much attention to the Tanwin and its other semantic implications, and have largely meant it to indicate indefinite nouns. In this study, it has been attempted to criticize and analyze the semantic aspects of the Tanwin and its different implications in some of the Quranic verses translation by Abdurrahman Pirani et al. The results show that paying attention to the semantic and implicating dimensions of the Tanwin can be an effective step towards enhancing the semantic levels of Quranic expressions and discovering the rhetorical-semantic layers of this divine book. But this translation, along with many of its more prominent features, does not pay much attention to the secondary meanings of the Tanwin, and this has led to some divergence from some of the sublime meanings and concepts in the Quranic vocabulary.

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In this study, 174 leaf samples from main apple growing areas in Kurdistan and western Azarbaijan Provinces were examined for infection by Apple chlorotic leaf spot virus (ACLSV). The result of RT-PCR showed that 17 samples (9.7%) were infected by ACLSV in both provinces. Subsequently, the genetic structure of ACLSV was determined based on the full coat protein (CP) sequences of eight isolates from this study and those recently published from Iran and worldwide. In phylogenetic analysis, ACLSV isolates from apple clustered into three known phylogenetic types; B6, P205, and SHZ, which are characterized by three amino acids at positions of 40, 75 and 79. The new Iranian ACLSV isolates showed the CP amino acid sequence of Ser⁴⁰-Tyr⁷⁵-Glu⁷⁹ which placed them with the B6 type. Further analyses showed that the Iranian ACLSV isolates (the new and previously published isolates) had lower genetic variability compared to isolates from other countries except for Brazilian population. Most of the CP codons were under negative selection (dN/dS < 1) except for one codon at position 92 which was under positive selection. This study suggests that negative selection was most important evolutionary factor driving the genetic evolution of ACLSV.
 

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Wrestling has been popular since ancient times and it has been associated with many aspects of Iranian culture. In this regard, wrestling has been a physical ritual, and today it is performed at the national, indigenous, and local levels. This study focuses on the myths behind this ritual movement. It is aimed to explain the myths that have been the reason for the meaning, continuity, and the value of this movement as a ritual in Iranian folk culture throughout history via the analytical method. In ancient culture, a person who fought with the destructive forces of nature became the heroic archetype who created livelihood and security in a society and was respected by the people. This challenge with the antagonist also entered the arena of the enemies of Iran, and wrestling was a symbol of the action of the heroes who fought a duel to protect his land and its values; but what was suggested in Iranian culture was that these heroes, whose actions establish the security and order of the society, first, must clear their background as an antagonist, and voluntarily go into the battle with shadows. In Iranian society, this became a value and moral foundation for the heroic archetype and remained in the collective subconscious of the Iranian society, and in the festivals of folk culture where wrestling is held, all these values are projected on the wrestler character. This forms the kind of social identity and cohesion through which viewers receive love and self-fulfillment.
Introduction
Research background
A few research projects been done on wrestling in folk culture. However, in these studies, types of wrestling in folk culture have been studied on a case. In the culture of every city and village, a type of native and local wrestling is common, and most studies have focused on one of these types such as Chokheh wrestling or Gilehmardi wrestling. Qasem Mehrabi et al. (2014) have dealt with the interaction of spectators and wrestlers of Chokheh, and Rahmati and Abbaszadeh (2016) have paid attention to the ability of Gilehmardi wrestling in the development of social participation. Since wrestling has a long history in Iranian culture, it is necessary to conduct research on the values and social beliefs that have led to the continuation of this national, indigenous, and local sport.
Aims, questions, and assumptions
The aim of the present research is to describe the mythology that has caused the continuity, meaning, and value of wrestling in Iranian society throughout history. Therefore, three theories could be indicated in this regard: Jung's archetypal, Campbell's mono-myth, and Maslow's hierarchy of needs to describe these myths through a qualitative descriptive-analytical method. The main question of this study is: what myths are behind this ritual movement activity? Wrestling is probably a movement activity that was intertwined with rituals and represented many myths through ritual performances.
 
 
Discussion
Archaeological evidence with the motif of wrestling and Persian mythology in the cultural context of ancient Iranian society shows that wrestling is a physical activity in a ritual that represents the heroism of prehistoric times that killed monsters to provide security for human life (Campbell, 2005). The monster is a symbol of some destructive natural forces that destroys the security and livelihood of people. The protagonist's combat with natural destructive forces, such as wild animals, becomes a physical habitus in the Iranian culture and takes on a ritual aspect. This ritual physical activity combines with other aspects of Iranian culture so that it is a show for the legitimacy of the king as a provider of security for the people and the wrestling is performed in the celebration of nature revitalization in the spring (Kaptain, 2013). Thus, the wrestling is a symbol of the battle of two opposing forces, one good, and the other evil. It is the ideology of the culture of ancient Iran that the force of good always triumphs over evil by choosing the right manner for battle (Rezai Rad, 2010). Folk literature also tells the story of wrestlers who wrestle with fair behavior, avoiding any inappropriate behavior. Thus, the wrestler is a symbol of the heroic archetype who removes individual and collective needs and has moral principles. The moral foundation of the Iranian hero archetype is Mithraism, at the top of which is the struggle against the inner shadow. The religion of Mithraism is a form of mysticism that teaches its followers the moral and social principles to make the world a better place (Cumont, 1966). According to these teachings of mysticism, the wrestler is also the protector of the social values.
Conclusion
Wrestling is a physical activity that is intertwined with the dimensions of Iranian culture. Iranian myths and beliefs give meaning to this physical activity and its integration with other cultural aspects. The wrestling is not only reminiscent of the hero archetypal actions of the prehistoric time but with its functions, it satisfies the psychological needs of the wrestler and the people. Hence, people project the character and moral qualities of ancient heroes on the wrestler. The inner shadow is the most important opponent of the wrestling, and by overcoming it, he can show the right manner in his behavior through wrestling.
 
References
Rahmati, M. M., & Abbaszadeh, M. (2016). Capability of rural sport; local development & social participation: a case study of Gilehmardi wrestling. Journal of Community Development, 7(1), 59-78.
Campbell, J. (2006). The hero of a thousand faces (translated into Farsi Shadi Khosrow Panah). Mashhad: GolAftab.
Cumont, F. (1966). The Mysteries of Mithra. Routledge.
Kaptan, D. (2014). Achaemenid history XII. The Daskyleion Bullae: seal images from the western Achaemenid empire (translated into Farsi Morteza. Saghebfar). Tehran: Toos.

Rezaei Rad, M. (2011). Fundamentals of political thought in Mazdaean wisdom (in Farsi). Tehran: TarheNou.

 

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Objective: Recent studies suggest that intermittent and prolonged normobaric hyperoxia (HO) results in ischemic tolerance to preventing ischemia brain injury. In this research attempts were made to see the changes in excitatory amio-acid transporter 3 (EAAT3), TNF-α levels, and NF-κB activity following prolonged and intermittent NBHO preconditioning. Materials and Methods: Rats were divided into four experimental groups, each with 21 animals. The first two groups were exposed to 95% inspired HO for 4h/day for 6 consecutive days (intermittent HO; InHO) or for 24 continuous hours (prolonged HO; PrHO). The second two groups acted as controls, and were exposed to 21% oxygen in the same chamber (normobaric normoxia, RA; room air) continuously for six days (intermittent RA, InRA) or for 24 hours (prolonged RA; PrRA). Each main group was subdivided to MCAO-operated (middle cerebral artery occlusion), sham-operated (without MCAO), and intact (without any surgery) subgroups. After 24h, MCAO-operated subgroups were subjected to 60min of right MCAO. After 24h reperfusion, neurologic deficit score (NDS) were assessed in MCAO-operated subgroups. Immediately and 48h after pretreatment, blood sampling for assessment of serum TNF- levels were purformed. Then, the effect of InHO and PrHO on serum TNF- levels, NF-κB activity and EAAT3 expression were measured. Results: Preconditioning with InHO and PrHO decreased NDS and upregulate EAAT3 and increase serum TNF-α level and NF-κB activity significantly. Conclusion: Although further studies are needed to clarify the mechanisms of ischemic tolerance, InHO and PrHO seems to partly exert their effects via increase in serum TNF-α levels, NF-κB activity and upregulation of glutamate transporters.

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Leptus (Leptus) molochinus (C. L. Koch, 1837) (Trombidiformes: Erythraeidae) was collected as an ectoparasite on Ophion sp. (Hymenoptera: Ichneumonidae), Paederus fuscipes Curtis, 1826 (Coleoptera: Staphylinidae), Cicadella viridis (L. 1758) (Hemiptera: Cicadellidae), Peritrechus sp. (Hemiptera: Lygaeidae); the ectoparasitic mite, Solistrus mitrae Saboori, Ueckermann & van Harten, 2008 was also found on an undetermined ant species (Hymenoptera: Formicidae) from Guilan province, Iran. Additional morphometric data for L. (L.) molochinus larvae are provided. Solistrus mitrae is reported for the second time in the world. Ophion sp. (Hymenoptera: Ichneumonidae), P. fuscipes (Coleoptera: Staphylinidae), C. viridis (Hemiptera: Cicadellidae) and Peritrechus sp. (Hemiptera: Lygaeidae) are recorded as new host taxa for L. (L.) molochinus and unknown ant species is new host for S. mitrae.

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In Archaeology the Iron Age refers to the period in which the immigration struggle intensified between different ethnic groups. The best data from these immigrant communities are their funerary structures. So that these structures have been seen in many areas of Archaeological where they are occupied. The most prominent funeral feature of these tribes that can be seen in the most entered lands is a circular stone structure on the grave that surrounds the central space or location of the dead. The study area is ​​one of the flooded rivers of Meshgin city that such a system is one of the important factors in the appearance of the ancient sites of this part of Ardabil province. In archaeological surveys, many Iron Age cemeteries were identified and studied in this area. One of the sensible Features for the tombs of these cemeteries is interesting circle-shaped structures that surround the inside of the grave and location of the dead place. This research is worked with brief background of the transmittal rate of such structures in the second and first millennium B.C graves in different parts of the world with looking for the mythological and symbology studies that why and how the circular structures are made in the ancient Iron Age burials of Meshgin Chayi basin. A sociological regard to this topic shows that there have been special belief between Iron Age societies that believe on those due to appearance and continuation of special methods in the dead interment

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Objective: Fungal nail infection (onychomycosis) is a common disease in all communities consisting about 50 percent of nail disorders. Yeasts are one of the important causative agents of onychomycosis. Identification of the yeast species is important in the epidemiological and therapeutical point of views. The aim of the present study is the precise species identification of the pathogenic yeast isolated from fungal nail infections, using the DNA-based methods. Materials and Methods: The isolates were preliminary studied according to study of morphological characteristics. For species identification, the genomic DNA of each sample was extracted by boiling method and the ITS region of ribosomal DNA was amplified by polymerase chain reaction (PCR). The amplified DNA was digested by the restriction enzyme MspI and each isolate was identified according to the electrophoretic patterns. A new enzymatic profile was used for final differentiation of Candida albicans and C. dubliniensis. A few of yeast isolates were identified by using ITS-sequensin. Results: C. albicans with the prevalence of 45.6% was the most common isolate, followed by C. parapsilosis with 22.5% and C. tropicalis with 21.8%. The less common species were C. glabrata, C. krusei, C. kefyr, C. lusitaniae, C. guilliermondii and C. pulcherrima that consisted 2.72%, 2%, 1.36%, 0.68% and 0.68% of the isolates, respectively. No C. dubliniensis was found among C. albicans isolates. Two isolates (1.36%) were identified as Trichosporon spp. The most common group of the patients was in the age range of 40-70 years old and the majority (83.2%) of the patients were women with finger nail infections. Conclusion: Although C. albicans is still the most prevalent isolates of nail candidiasis, the increasing number of non-albicans species is notable. The study showed that for identification of some rare species, the routine phenotypical approaches are not efficient and application of the ITS-PCR-RFLP can improve the level of differentiation up to 98%. The remaining isolates can be identified by more expensive methods such as sequencing.

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All species in the genus Lysiphlebus Förster (Hymenoptera: Braconidae, Aphidiinae) are solitary endoparasitoids of aphids, which are one of the most difficult and unknown taxonomic groups to identify. A pair of species-specific primers as a molecular marker was designed to identify the most abundant and important aphid parasitoid wasp, L. fabarum Marshall, collected from Guilan province (Iran). This primer pair, which produces a 148 bp fragment length, was developed for L. fabarum by using the ATPase 6 gene region of mitochondrial DNA. The lower detection limit to amplify DNA of L. fabarum in a singleplex PCR was determined to be 72 pg of parasitoid DNA/µl PCR which was enough to detect this parasitoid in early life stages within its host. The L. fabarum eggs cannot be detected within Aphis fabae Scopoli (Hemiptera: Aphididae) earlier than 12 hours after oviposition. A pair of species specific primers designed for L. fabarum can be used to monitor the wasp populations under field conditions.