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The family Neanuridae belonging to order Poduromorpha, class Collembola includes terrestrial animals which play vital roles in decomposition and nutrient cycle in soil. Up to now, the fauna of this family has not been investigated in various areas of Golestan national Park (Hyrcanian forests). In order to identifying species of the class Collembola in this park, numerous samplings were carried out from soil, humus, and moss in 2015 and 2016. Then, the Collembola specimens were separated by using Berlese Funnel, stored in 75-85 percent ethyl alcohol and identified according to valid identification keys. Meanwhile, the species Friesea afurcata Tullberg, 1869) from the Neanuridae family is reported for the first time in Iran and Golestan province.