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Background: Due to the side effects of chemical and synthetic antibiotics and the increase in bacterial resistance, extensive research has been conducted to obtain natural compounds without side effects from natural sources such as insects, especially Diptera order, because these insects require to live and survive on rotting food and be in direct contact with pathogenic and dangerous microorganisms because of their special diet.
Materials & Methods: In this study, hemolymphs were extracted from Musca domestica, Drosophila melanogaster, and Sarcophaga nodosa, and then the anti-bactericidal activity of these extracts against important pathogenic bacteria was investigated separately by the minimum inhibitory concentration (MIC) method using resazurin indicator.
Findings: S. nodosa and M. domestica larvae were not able to prevent the growth of any of the bacteria. D. melanogaster larvae extract completely inhibited the growth of Escherichia coli and Pseudomonas aeruginosa bacteria at all densities, while Staphylococcus aureus was completely resistant to all concentrations. The minimum inhibitory concentration of D. melanogaster larvae extract against two bacteria of Listeria monocytogenes and Salmonella typhimurium was determined as 125 and 500  μL/mL, respectively.
D. melanogaster pupae extract was unable to inhibit the growth of E. coli and S. typhimurium but prevented the growth of P. aeruginosa at all concentrations. Also, the minimum inhibitory concentration of this extract against both S. aureus and L. monocytogenes was determined as 1000 μL/mL.
Conclusion: These outcomes show that D. melanogaster holds a high potential of antibacterial effects, and the purification and evaluation of this extract active substances are recommended for future utilization as antibacterial agents and food preservatives to fight pathogenic and toxigenic microorganisms.

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The population assemblage of Drosophilidae in the four districts of North Western Ghats was analysed. A total of 13,604 individuals comprising 17 species collected from 8 localities during the period of 2021–22 across various seasons. The study highlights the dominance of three species (Drosophila bipectinata Duda, D. malerkotliana Parshad & Paika and D. eugracilis Bock & Wheeler) belonging to ananassae and eugracilis subgroups. Overall collection data revealed the highest species richness and diversity for Dharwad and UK interior forests, whereas the maximum abundance and the highest evenness were observed in UK coastal and Belagavi forests respectively. The species rank-abundance curve revealed Dharwad forest had higher species richness and comparatively stable species assemblage. Drosophila eugracilis was the dominant species in localities of Dharwad and Belagavi forests, whereas D. bipectinata was the dominant species in both coastal and interior localities of UK forests. Rare faction curves plotted across the different seasons for all the forest localities revealed population assemblage and species richness of all forests across different seasons. Morisita index of similarities showed similarities for populations across localities and seasons. Nonparametric independent sample Kruskal-Wallis test was done to test distribution of abundance of individual species across spatial and temporal groups. The study reveals variation of population assemblage across the forests of Dharwad, Belagavi, and Uttara Kannada (coastal and interior) and dominance of D. bipectinata, D. malerkotliana and D. eugracilis.