Volume 6, Issue 4 (2020)                   J. Insect Biodivers. Syst 2020, 6(4): 331-342 | Back to browse issues page


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Pasandideh Saqalaksari M, Talebi A A, van de Kamp T. MicroCT 3D reconstruction of three described braconid species (Hymenoptera: Braconidae). J. Insect Biodivers. Syst. 2020; 6 (4) :331-342
URL: http://jibs.modares.ac.ir/article-36-40911-en.html
1- Department of Agricultural Entomology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
2- Department of Agricultural Entomology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran , talebia@modares.ac.ir
3- Laboratory for Applications of Synchrotron Radiation (LAS), Karlsruhe Institute of Technology (KIT), Kaiserstr. 12, 76131, Karlsruhe, Germany
Abstract:   (1313 Views)
Traditionally, entomologists have used destructive methods especially dissection in order to investigate the important taxonomic characters of specimens. New technologies for imaging and analyzing in taxonomy, offer opportunities to deposit three-dimensional (3D) data to proposed for rare and valuable type materials in museums and collections. Micro-computed tomography, as a non-destructive imaging technique, has become an emerging and progressive technology in insect science. However, this technology is rarely used in entomology compared to in medical and industrial applications. In this study, MicroCT imaging protocols are explained in detail using three species of braconid wasps: Aleiodes arnoldii Tobias, 1976 (Braconidae: Rogadinae), Hormius moniliatus Nee, 1811 (Braconidae: Hormiinae) and Macrocentrus bicolor Curtis, 1833 (Braconidae: Macrocentrinae). MicroCT scan data of three braconid wasp species from Iran, depicted main identification of skeletal body parts. A brief step-by-step is provided on image acquisition, 3D reconstruction and mesh editing to create a virtual model of the species utilized for morphological and morphometric studies. As a result, the use of micro-computed tomography as a non-invasive virtual examination tool was explored. The complete datasets containing the raw TIFF MicroCT data, 3D models and 3D rotation videos available for download at http://www.morphosource.org/Detail/ProjectDetail/Show/project_id/822
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Article Type: Research Article | Subject: Systematics/Hymenoptera
Received: 2020/05/11 | Accepted: 2020/07/11 | Published: 2020/07/23

References
1. Akkari, N., Enghoff, H. & Metscher, B.D. (2015) A new dimension in documenting new species: high-detail imaging for myriapod taxonomy and first 3D cybertype of a new millipede species (Diplopoda, Julida, Julidae). PLoS One, 10 (8), e0135243. [DOI]
2. Akkari, N., Ganske, A.S., Komerički, A. & Metscher, B. (2018) New avatars for myriapods: Complete 3D morphology of type specimens transcends conventional species description (Myriapoda, Chilopoda). PLoS One, 13, 1–12. [DOI]
3. Ameri, A., Talebi, A.A., Rakhshani, E. & Beyarslan, A. (2016) A Review of the Subfamily Hormiinae (Hymenoptera: Braconidae) from Iran. Journal of Insect Biodiversity and Systematics, 1 (2), 111–123.
4. Beutel, R.G., Friedrich, F. & Aspöck, U. (2010) The larval head of Nevrorthidae and the phylogeny of Neuroptera (Insecta). Zoological Journal of Linnean Society, 158 (3), 533–562. [DOI]
5. Beutel, R.G., Ge, S. & Hörnschemeyer, T. (2008) On the head morphology of Tetraphalerus, the phylogeny of Archostemata and the basal branching events in Coleoptera. Cladistics, 24, 270–298.
6. Boring, C.A., Sharkey, M.J. & Nychka, J.A. (2009) Structure and functional morphology of the ovipositor of Homolobus truncator (hymenoptera: Ichneumonoidea: Braconidae). Journal of Hymenoptera Research, 18, 1–24.
7. Curtis, J. (1833) Characters of some undescribed genera and species indicated in the "Guide to an arrangement of British insects. Entomological Magazine, 1, 186–199.
8. Farahani, S., Talebi, A.A. & Rakhshani, E. (2012) First records of Macrocentrus Curtis, 1833 (Hymenoptera: Braconidae: Macrocentrinae) from Northern Iran. Zoology and Ecology, 22 (1), 41–50. [DOI]
9. Farahani, S., Talebi, A.A., van Achterberg, C. & Rakhshani, E. (2015) A review of the subfamily Rogadinae (Hymenoptera: Braconidae) from Iran. Zootaxa, 3973 (2), 227–250. [DOI]
10. Faulwetter, S., Vasileiadou, A., Kouratoras, M., Dailianis, T. & Arvanitidis, C. (2013) Micro-computed tomography : Introducing new dimensions to taxonomy. Zookeys, 263, 1–45. [DOI]
11. Fischer, G., Sarnat, E.M. & Economo, E.P. (2016) Revision and Microtomography of the Pheidole knowlesi Group, an Endemic Ant Radiation in Fiji (Hymenoptera, Formicidae, Myrmicinae). PLoS One, 11 (8), e0161520. [DOI]
12. Friedrich, F. & Beutel, R.G. (2008) Micro-computer tomography and a renaissance of insect morphology. Proceedings of Society of Photo-Optical Instrumentation Engineers (SPIE) 7078, Developments in X-Ray Tomography VI. 70781U., San Diego, California, USA.
13. Friedrich, F., Matsumura, Y., Pohl, H., Bai, M., Hörnschemeyer, T. & Beutel, R.G. (2014) Insect morphology in the age of phylogenomics: Innovative techniques and its future role in systematics. Entomological Science, 17 (1), 1–24. [DOI]
14. Garcia, F.H., Fischer, G., Liu, C., Audisio, T.L., Alpert, G.D., Fisher, B.L. & Economo, E.P. (2017) X-Ray microtomography for ant taxonomy: An exploration and case study with two new Terataner (Hymenoptera, Formicidae, Myrmicinae) species from Madagascar. PLoS One, 12 (3), e0172641. [DOI]
15. Godfray, H.C.J. (2007) Linnaeus in the information age. Nature, 446 (7133), 259–260. [DOI]
16. Greco, M., Jones, A., Spooner-Hart, R. & Holford, P. (2008) X-ray computerised microtomography (MicroCT): a new technique for assessing external and internal morphology of bees. Journal of Apicultural Research, 47 (4), 286–291. [DOI]
17. Hörnschemeyer, T., Beutel, R.G. & Pasop, F. (2002) Head structures of Priacma serrata leconte (coleptera, archostemata) inferred from X‐ray tomography. Journal of Morphology, 252, 298–314. [DOI]
18. Iwan, D., Kamiński, M.J. & Raś, M. (2015) The Last Breath: A μCT-based method for investigating the tracheal system in Hexapoda. Arthropod structure & Development, 44, 218–227. [DOI]
19. Lipke, E., Ramírez, M.J. & Michalik, P. (2014) Ultrastructure of spermatozoa of orsolobidae (Haplogynae, Araneae) with implications on the evolution of sperm transfer forms in Dysderoidea. Journal of Morphology, 275 (11), 1238–1257. [DOI]
20. Metscher, B.D. (2009) MicroCT for developmental biology: A versatile tool for high-contrast 3D imaging at histological resolutions. Developmental Dynamics, 238, 632–640. [DOI]
21. Nees von Esenbeck, C.G. (1811) Ichneumonides adsciti in genera et familias divisi a Dre. Nees von Esenbeck. Magazin Gesellschaft Naturforschender Freunde zu Berlin 5, 3–37
22. Pasandideh Saqalaksari, M., Talebi, A.A. & van de Kamp, T. (2020) Application of synchrotron Micro-computed tomography in assessing external anatomy of Aleiodes arnoldii (Hymenoptera: Braconidae). Annual International Congress of New Findings in Agricultural Sciences and Natural Resources, Environment and Tourism. 15 February 2020. Tehran, Iran, p. 1–6.
23. Quicke, D.L.J. (2015) The Braconid and Ichneumonid Parasitoid Wasps: Biology, Systematics, Evolution and Ecology. John Wiley & Sons, Ltd., Chichester, UK, 704 pp.
24. Rahman, M.H., Fitton, M.G. & Quickei, D.L.J. (1998) Ovipositor internal microsculpture in the Braconidae (Insecta, Hymenoptera). Zoologica Scripta, 27 (4), 319–331. [DOI]
25. Richards, C.S., Simonsen, T.J., Abel, R.L., Hall, M.J.R., Schwyn, D.A. & Wicklein, M. (2012) Virtual forensic entomology: Improving estimates of minimum post-mortem interval with 3D micro-computed tomography. Forensic Science International, 220 (1–3), 251–264. [DOI]
26. Sarnat, E.M., Friedman, N.R., Fischer, G., Lecroq-Bennet, B. & Economo, E.P. (2017) Rise of the spiny ants: diversification, ecology and function of extreme traits in the hyperdiverse genus Pheidole (Hymenoptera: Formicidae). Biological Journal of the Linnean Society, 514–538. [DOI]
27. Sharkey, M. J. & Wharton, R.A. (1997) Morphology and Terminology. In: Wharton, R.A., Marsh, P.M. & Sharkey, M.J. (eds.) Manual of the New World genera of the family Braconidae (Hymenoptera), Special Publication of the International Society of Hymenopterists. No 1, Washington DC, pp. 19–37.
28. Shaw, M.R. & Huddleston, T. (1991) Classification and biology of braconid wasps (Hymenoptera: Braconidae). Handbooks for the Identification of British Insects. Royal Entomological Society of London, London, UK, 126 pp.
29. Simonsen, T.J. & Kitching, I.J. (2014) Virtual dissections through micro‐CT scanning: a method for non‐destructive genitalia ‘dissections’ of valuable L epidoptera material. Systematic Entomology, 39, 606–618. [DOI]
30. Tobias, V.I. (1976) Braconids of the Caucasus (Hymenoptera, Braconidae). Opred. Faune SSSR. 110. Nauka Press, Leningrad, 296 pp.
31. van de Kamp, T., Schwermann, A.H., dos Santos Rolo, T., Lösel, P.D., Engler, T., Etter, W., Faragó, T., Göttlicher, J., Heuveline, V., Kopmann, A., Mähler, B., Mörs, T., Odar, J., Rust, J., Tan Jerome, N., Vogelgesang, M., Baumbach, T. & Krogmann, L. (2018) Parasitoid biology preserved in mineralized fossils. Nature Communications, 9, 3325. [DOI]
32. Wharton, R.A., Shaw, S.R., Sharkey, M.J., Wahl, D.B., Woolley, J.B., Whitfield, J.B., Marsh, P.M. & Johnson, W. (1992) Phylogeny of the Subfamilies of the Family Braconidae (Hymenoptera: Ichneumonoidea): a Reassessment. Cladistics, 8 (3), 199–235. [DOI]
33. Wojcieszek, J.M., Austin, P., Harvey, M.S. & Simmons, L.W. (2012) Micro‐CT scanning provides insight into the functional morphology of millipede genitalia. Journal of Zoology, 287 (2), 91–95. [DOI]
34. Yu, D.S., Achterberg, C. van, Horstmann, K. & van Achterberg, K. (2016) Taxapad 2016, Ichneumonoidea 2015. Database on flash-drive. Ottawa, Ontario, Canada.
35. Zimmermann, D., Randolf, S., Metscher, B.D. & Aspöck, U. (2011) The function and phylogenetic implications of the tentorium in adult Neuroptera (Insecta). Arthropod Structure & Development, 40 (6), 571–582. [DOI]

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